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Journal of Stem Cell Research and Tissue Engineering
Published by Universitas Airlangga
ISSN : 26141264     EISSN : 26141256     DOI : https://dx.doi.org/10.20473/jscrte
Core Subject : Health, Science, Engineering,
Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary
Journal of Stem Cell Research and Tissue Engineering (JSCRTE) is published by Stem Cell Research and Development Center, Airlangga University. Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. Special focus of JSCRTE is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. Articles that go through the selection process will be review by peer reviewer or editor. The journal is published regularly twice a year in December and May. Every publication consists of 60-70 pages and 5 scientific articles in the form of research, study literature, and the case study in English. The contributors Journal of Stem Cell Research and Tissue Engineering are Stem Cell researchers, lecturers, student and practitioners that came from Indonesia and abroad.
Arjuna Subject : Kedokteran - Imonologi dan Alergi
Articles 5 Documents
 1 
Search results for , issue "Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING" : 5 Documents clear
Genetic Stability The Protein Encoding Envelope (E) Genes Dengue Virus Serotype-4 Passaged in Vero Cell As A Candidate Chimera Vaccine Material deya karsari
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22748

Abstract

This study aims to  analyze   genetic stability of  the gene encoding the envelope protein (E) dengue virus serotype-4 passaged in vero cells, Denv-4 passaged  in vero cells serially then continued with RNA extraction at passage 0, 10, 20, 30, 40 ,50 , and 60, and then continued with two step PCR and amplification, and sequencing then analyze the nucleotide stability with BLAST and MEGA 5 software. The result shows that there are many variable site in nucleotide and amino acid with high mutation rate 57.4% for nucleotide and 71.9% for amino acid ,while the similarity between passages are high ranging from 91% - 98%. The conclusion for this study is Denv-4 after analyzed shows that the gene encoding protein E has many variable site but high in similarity.
The Therapy of Burn Wound Healing in Rat (Wistar) by Using the Combination of Peripheral Blood Mononuclear Cells (PBMCs) and Bone Marroe BM-Derived Mesenchymal Stem Cell Gusti Revilla
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22753

Abstract

The therapy to heal the bum wound is still imperfect, therefore it is important to conduct specific research concerning this topic that benefits to society. Using both Bone Marrow-stem cell (BM-MSc) and peripheral blood mononuclear cells (PBMCs) from allogenic donors as part of the therapy to heal the bum wound seems to give positive prospect for the future treatment. In this experiment, PBMC and rat BM- derived from mesenchymal stem cell were used as the therapy model. lmmunocytochemistry was used as the method to characterize the phenotype of MSc, It was also used to express the collagen type I and the Indirect ELISA in analyzing the TGF·P 1 secretion.  The rats with bum wound were divided into 2 kinds of group; the first group of rats was selected to control the use of PBS; while second group of rats was used as the treatment object that was medicated by the applying of the combination of both BM-MSC and PBMC. Stem-cells subcutaneously administered dose applied to each rat was around of 2 x 106 cells. The result showed that the levels of TGF-β1 secretion in day 3rd and day 7th on the rats which were treated by using the combination of BM-MSC and PBMC were higher compared to the rats from the control group.  The experiment that concerns on the thickness of the collagen showed that combination between BM-MSC and PBMC stem cell make it possible in increasing the thickness of collagen 1, besides; it also showed significant differences (p=0.000). This research proved that the combination of BM-MSC and PBMC stem cell served can accelerate the healing process for the bum wound on rats through Increasing of TGF-P 1 secretion and collagen type 1 expression, It means that PBMCs can be applied as good as chemoattractant.
Characterization of CD4+ Lymphocyte From Bone Marrow Stem Cell Using Indirect Immunofluoresence For HIV & AIDS Treatment Purwati Purwati
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22749

Abstract

Acquired immune deficiency syndrome (AIDS) is caused by Human Immunodeficiency Virus (HN). At the beginning of infection, gpl20 virus interacts with CD4 receptor at the surface of the target cell. The interaction between gpI20 and CD4 leads to the occurrence of the binding of specific chemokine receptor CXCR4 and CCR5, which are also present on the membrane of the target cell.  Therefore, CCR5 arid CXCR4 also determine the fate of the target cell.  It is the performance   of CCR5 and CXCR4, guided by controlling gene that determines susceptibility or resistance to HN infection.  Coding gene CCR5 may mutate to become protective or resistant against HTV infection. In homozygote   individuals, it tends to be resistant against infection while in heterozygote individuals it tends to be susceptible to HN infection.  Objective:  To characterize   TCD4 lymphocyte in the next that is resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HTV & AIDS treatment. Method: Sample collection, mononucleated cell collection, lymphocyte culture, CD4 identification, CCR5 variance analysis, co-cultivation with PBMC HN and comparison to control. Result:  This study was performed in several steps, such as mononucleated   cell isolation, followed with cell culture, lymphocyte purification, lymphocyte and CD4 expression identification.   Conclusion:  Lymphocyte T CD4 had been mature after seven passages, once passage is about 5 days so for maturity lymphocyte T CD4 need 35 days and that cell as be candidate to resistant against HN infection by using gene therapy deletion 32 CCR5 to use for HN   & AIDS treatment.
The Effect of Bone Marrow Transplantation on Oocyte-Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat hendi hendarto
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22751

Abstract

Introduction: Chemotherapy has cytotoxic effect that induces follicular damage and abnormal folliculogenesis leads to ovarian failure. Two crucial   growth factors in abnormal folliculogenesis, Growth Differentiation Factor-9 (GDF-9) and Kit-Ligand, will be disrupted and affect follicular development. In this study, we evaluate whether bone marrow transplantation (BMT) has a role on oocyte-granulosa cell interaction by analyzing GDF-9 and Kit-Ligand expressions and also follicular development by analyzing primordial, primary, secondary and graafian follicles of cisplatin-induced ovarian failure in rat. Material and method: Forty eight rats were divided into three groups: control, cisplatin and cisplatin + BMT. Ovarian failure was induced by administration   of intraperitoneal cisplatin 5 mg/kg body weight for 1 week. BMT 2 x107 cells were injected through rat tail vein after cisplatin administration.  Bone marrow was isolated from rat femur and characterized    by CD44(+), CD45(-), CD105(+). Immunohistochemistry examinations for ovarian GDF-9, Kit-Ligand and follicle development evaluation were performed after 2 weeks of BMT injection. Results:  The  expressions   of Kit-ligand  among  three  groups  by ANOVA were  significant different (p=0.00), whereas by Post Hoc: cisplatin group lower  than  control  group (p=0.00); cisplatin + BMT group  higher than  cisplatin group (p=0.00); and no significant different between  control  group and cisplatin + BMT group (p=0.955). The expressions of GDF-9 by Kruskal Wallis showed significant different (p=0.00) among three groups whereas cisplatin + BMT group higher than cisplatin group and control group. In cisplatin + BMT group the number of primordial, primary, secondary and graafian follicles were higher than those in cisplatin group; but were lower than those in control group (p=0.000). Positive Paul Kart Horan (PKH) labeling was seen in cisplatin + BMT   group only. Conclusion:  In cisplatin-induced ovarian failure in rat, bone marrow transplantation may improve oocytegranulosa cell interaction and follicular development.
The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro erma safitri
Journal of Stem Cell Research and Tissue Engineering Vol. 4 No. 2 (2020): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v4i2.22752

Abstract

The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).

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